This research is aimed to study the distribution of tropopause folding frequency and its seasonal changes over Southwest Asia, for a 5-year period from 2011 up to 2015. Identification of tropopause folding is based on the algorithm developed by Škerlak et al. (2014) using pseudo soundings in each of the grid points. Since tropopause folding occurs mainly in the area with strong horizontal temperature gradient and vertical wind shear, tropopause folding in Southwest Asia forms near the subtropical jet stream. In the summer season, as the monsoon anticyclone is formed over the subtropical region of the Indian Ocean, the folding frequency increases substantially. Two regions with the maximum value of the folding frequency are seen over Iran–Afghanistan and the eastern of the Mediterranean Sea during the summer. Also, the value of folding frequency, especially the medium and deep types, is increased remarkably in the winter season. This can be attributed to baroclinic instability event, because both the number and intensity of the event is increased in the winter.