Land subsidence cause damage to urban buildings and facilities and increase their seismic vulnerability. This hazard in Isfahan metropolitan has caused shear and tensile cracks in buildings, displacement of infilled frames, rotation of structures and change their separation joints. In these structures, story drift and P-Delta effect increase during earthquakes and adjacent buildings collide due to decrease separation joint. These structures cannot tolerate base acceleration of design and moderate earthquakes. Increase of sinkholes in Isfahan maybe due to land subsidence fissures in urban areas and asymmetric settlement. During earthquakes, many sinkholes form and will block relief routes, cut gas pipelines and cause widespread fires. Considering the seismic history of this region, it is necessary to consider special engineering methods for effects of land subsidence in design of buildings and urban development.